https://nova.newcastle.edu.au/vital/access/ /manager/Index ${session.getAttribute("locale")} 5 Short-duration hypothermia induction in rats using models for studies examining clinical relevance and mechanisms https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:39601 Wed 29 Mar 2023 13:54:42 AEDT ]]> Comparison of three different methods for determining cell proliferation in breast cancer cell lines https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:30103 in vitro including conventional hemocytometer counting chamber, a luminescence-based assay that utilizes the change in the metabolic activity of viable cells as a measure of the relative number of cells, and a multi-mode cell imager that measures cell number using a counting algorithm. Each method presents its own advantages and disadvantages for the measurement of cell proliferation, including time, cost and high-throughput compatibility. This protocol demonstrates that each method could accurately measure cell proliferation over time, and was sensitive to detect growth at differing cellular densities. Additionally, measurement of cell proliferation using a cell imager was able to provide further information such as morphology, confluence and allowed for a continual monitoring of cell proliferation over time. In conclusion, each method is capable of measuring cell proliferation, but the chosen method is user-dependent.]]> Wed 17 Nov 2021 16:31:24 AEDT ]]> Epidural intracranial pressure measurement in rats using a fiber-optic pressure transducer https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:13751 Wed 11 Apr 2018 12:40:01 AEST ]]> Organ culture and whole mount immunofluorescence staining of mouse Wolffian ducts https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:30902 Wed 04 Sep 2019 09:54:17 AEST ]]> Recording Network Activity in Spinal Nociceptive Circuits using Microelectrode Arrays https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:47016 Tue 13 Dec 2022 12:23:37 AEDT ]]> Analysis of epididymal protein synthesis and secretion https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:34868 Thu 30 May 2019 15:31:33 AEST ]]> A simple migration/invasion workflow using an automated live-cell imager https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:36559 Thu 28 Oct 2021 13:03:29 AEDT ]]> Phosphopeptide analysis of rodent epididymal spermatozoa https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:19261 Sat 24 Mar 2018 08:06:38 AEDT ]]> Excitotoxic stimulation of brain microslices as an in vitro model of stroke https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:17047 Sat 24 Mar 2018 08:05:06 AEDT ]]> Ex vivo intestinal sacs to assess mucosal permeability in models of gastrointestinal disease https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:29359 Sat 24 Mar 2018 07:34:17 AEDT ]]> The emotional stroop task: assessing cognitive performance under exposure to emotional content https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:29290 Sat 24 Mar 2018 07:32:53 AEDT ]]>